Fig 1: m6A regulation at synapses is activity-time dependent and involves m6A demethylation.RNA binding proteins change in proximity to modified transcripts after synaptic stimulation as imaged by confocal microscopy. A 15 min after NMDA activation of dSH-SY5Y cells induces increased colocalisation between m6A-RNAs (green), the eraser protein ALKBH5 (red) and post synaptic marker, PSD-95 (magenta). Insets of selected regions are shown on the right. B Experimental design. Mean Pearson’s Correlation Coefficient for m6A colocalisation with YTHDF1 (C), YTHDF3 (D), Dcp1A (E), ALKBH5 (F) and FMRP (G) at postsynaptic sites at 15 min or 24 h after NMDA and KCl application in dSH-SY5Y cells. Error bars denote 95% CI. *p ≤ 0.05, **p ≤ 0.005, ***p ≤ 0.0005, ****p ≤ 0.00005.